Linkage and association analysis of hyperthyrotropinaemia in an Alpine population reveal two novel loci on chromosomes 3q28-29 and 6q26-27

To identify genes contributing to hyperthyrotropinemia and clinical hypothyroidism, we performed linkage analysis on 1,258 individuals from three semi-isolated Alpine villages. Cases were defined based on TSH levels (TSH ≥ 4.6 mU/L and TSH > 3.0 mU/L) and medical treatment while having normal/low fT4 levels. Two peaks on chromosomes 3q28-29 and 6q26-27 were identified. SNP- […]

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Detection of APC germ line mosaicism in patients with de novo familial adenomatous polyposis: a plea for the PTT

Current recommendations for APC mutation analysis in patients with familial adenomatous polyposis (FAP) advise full gene sequencing and gene dosage analysis replacing the protein truncation test (PTT) as a pre-screening tool. Here we report on two unrelated patients with classical FAP and unremarkable family history in whose leukocyte-derived DNA no pathogenic APC mutations could be […]

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Predicting PTEN mutations: an evaluation of Cowden syndrome and Bannayan–Riley–Ruvalcaba syndrome clinical features

Cowden syndrome (CS) is an important hereditary cancer syndrome, is caused by mutations in the PTEN gene, which causes significantly increased risks for breast, thyroid, uterine and perhaps colon cancers.  Since 1996 the clinical diagnosis of CS has been based upon criteria established by a research consortium. These criteria, however, have not been rigorously examined […]

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NLRP7 in the spectrum of reproductive wastage: rare non-synonymous variants confer genetic susceptibility to recurrent reproductive wastage

Mutations in NLRP7, a gene with a predicted role in inflammation and apoptosis, are responsible for recurrent molar pregnancies. In this study we report NLRP7 mutations in women with sporadic non-recurrent moles and in women with recurrent spontaneous abortions and no moles. We show that some rare NLRP7 variants, present in the general population, are […]

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Detection of APC germ line mosaicism in patients with de novo familial adenomatous polyposis: a plea for the PTT

Current recommendations for APC mutation analysis in patients with familial adenomatous polyposis (FAP) advise full gene sequencing and gene dosage analysis replacing the protein truncation test (PTT) as a pre-screening tool. Here we report on two unrelated patients with classical FAP and unremarkable family history in whose leukocyte-derived DNA no pathogenic APC mutations could be […]

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Replacement of the myotonic dystrophy type 1 CTG repeat with “non-CTG repeat” insertions in specific tissues

One of the most mutation-prone sequences is the variable number of repeating DNAs at the myotonic dystrophy (DM1) gene, causing muscular dystrophy.  In DM1 individuals the mutant gene has an expansion of a repeated DNA sequence CTG CTG CTG to hundreds or thousands of repeats.  We report a new kind of mutation at the DM1 […]

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