One in three highly selected Greek breast cancer patients carries a loss-of-function variant in a cancer susceptibility gene

Pathogenic variants in a number of genes, conferring high or moderate risks have been associated to hereditary breast cancer. Studying a highly selected cohort of 1,382 Greek breast cancer patients, 436 inherited pathogenic variants distributed in twenty-eight genes, have been identified. Beyond established associations, i.e. high breast cancer risk conferred by TP53 and PALB2 pathogenic […]

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Clinical features and cancer risk in families with pathogenic CDH1 variants irrespective of clinical criteria

Individuals with mutations in the CDH1 gene have the inherited hereditary diffuse gastric cancer syndrome. This study is the first report on cancer risk in patients with CDH1 mutations that were not previously selected from families highly suspicious for this syndrome. The results indicate that gastric cancer risks are lower and age at diagnosis higher […]

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Genetic characteristics of retinitis pigmentosa in 1,204 Japanese patients

Retinitis pigmentosa (RP) is the most common form of hereditary retinal degenerative disease worldwide. We performed large-scale high-coverage targeted resequencing of 83 causative genes of RP. The analyzed samples accounted for more than 4 % of all RP patients in Japan, leading us to reveal comprehensive characteristics of causative genes and variants in the Japanese […]

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Distal chromosome 16p11.2 duplications containing SH2B1 in patients with scoliosis

In this article, the authors test for enrichment of copy number variants (CNVs) at 55 well-characterized hotspots of recurrent copy number variation within a large cohort of isolated adolescent idiopathic scoliosis (AIS) patients. Of these 55 hotspots, only distal 16p11.2 duplications were enriched in AIS cases compared to control individuals. The authors replicated this finding […]

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Pathogenic variants in PLOD3 result in a Stickler syndrome-like connective tissue disorder with vascular complications

Classification of rare, multisystemic connective tissue disorders (CTDs) can be difficult due to overlapping features with multiple CTDs and scarcity of clinical information. We describe 3 individuals in one family with a rarely reported autosomal recessive CTD due to pathogenic variants in PLOD3 that encodes the lysyl hydroxylase 3 enzyme, important for post-translational collagen modification.  […]

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Gastric cancer in Lynch syndrome is associated with underlying immune gastritis

The factors that predispose individuals with Lynch syndrome to gastric cancer are not well-described.  In our cohort of 255 individuals with Lynch syndrome, chronic immune gastritis was observed in over 70% of the cases with gastric cancer and in none of the patients without gastric cancer.  This suggests that chronic inflammation is an important risk […]

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Homozygous mutations in SPEF2 induce multiple morphological abnormalities of the sperm flagella and male infertility

Male infertility due to multiple morphological abnormalities of the sperm flagella (MMAF) is a genetically heterogeneous disorder. Previous studies revealed several MMAF-associated genes, which account for approximately 60% of human MMAF cases. The pathogenic mechanisms of MMAF remain to be illuminated. In this study, we identified SPEF2 as a novel gene for human MMAF across […]

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Prenatal ultrasound findings of rasopathies in a cohort of 424 fetuses: update on genetic testing in the NGS era

In this study we describe the prenatal characteristics of Noonan syndrome and its associated syndromes called Rasopathies in 40 fetuses with a pathogenic variant in one of the Rasopathy genes. The most common feature is increased nuchal translucency (NT). Based on our data we suggest genetic testing of fetuses with an isolated persistent NT ≥5.0 […]

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Bi-allelic loss of function variants of TBX6 causes a spectrum of malformation of spine and rib including congenital scoliosis and spondylocostal dysostosis

Congenital scoliosis (CS) is a common spinal anomaly and spondylo-costal dysostosis (SCDO) is a rare skeletal dysplasia affecting spine and rib. They have been considered to belong to different disease entities. We examined TBX6 in CS and SCDO and found loss-of-function (LOF) mutations in both. There were two LOF mechanisms: decreased transcriptional activity and mis-localization […]

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